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Ultra Fine-Tuned™ technology is what sets the Synergy Mx Multi-Mode Reader apart. Its quadruple monochromator system selects wavelengths with a repeatability of plus or minus 0.2 nm. Its top optical head can focus up and down on the samples with a 100 µm resolution. Four slits on the excitation and emission side provide a choice of 16 bandpass combinations for every wavelength pair. Its advanced 4-Zone™ temperature control system incubates up to 65°C with a precision of ± 0.5°C at 37°C. These unique features and BioTek’s exclusive focus on microplate instrumentation and software make the Synergy Mx the most precise, sensitive and flexible multi-mode microplate reader available today for life-science research applications.


- Fully monochromator-based with quadruple grating architecture for Ultra Fine-Tuned performance.

- Compatible with Take3 Multi-Volume Plate: sixteen 2 µL microspots for low volume DNA/RNA 260 nm measurements. Saves precious samples, very fast and simple process.

- High performance fluorescence (top and bottom), absorbance and dedicated luminescence detection systems accommodate all life-science research microplate applications.

- Variable bandpass selection adds flexibility in fluorescence mode.

- Automated Z-height adjustment accommodates variable microplate heights and liquid levels with 100 μm resolution.

- Cuvette port to measure standard 1-cm path cuvettes if only a few samples need to be run, or to compare data with a standard cuvette spectrophotometer.

- Modular architecture allows purchase of required modes with the ability to upgrade later.

- Optional dual-reagent dispenser can be used for assays requiring precise timing such as ion channel assays or luminescent flash ATP assays.



Detection methodFluorescence, UV-Visible Absorbance, Luminescence, Time-Resolved Fluorescence (secondary mode)
Read methodEnd point, kinetic, spectral scanning, well-area scanning
Microplate types1- to 384-well plates and PCR plates
Compatible with Take3 Multi-Volume Plate with 2 µL microspots
Temperature control4°C above ambient to 65°C +0.5°C at 37°C
SoftwareGen5™ included
Control through USB or serial port



Light sourceHigh Energy Xenon Flash
Wavelength selectionDouble Monochromator
Wavelength range230 - 999 nm, 1 nm increment
Bandpass2 nm (230-285 nm), 4 nm (>285 nm)
Dynamic range0 - 4.0 OD
Resolution0.0001 OD
Pathlength correctionYes
Monochromator wavelength accuracy+2 nm
Monochromator wavelength repeatability+0.2 nm
OD accuracy<1% at 2.0 OD typical
OD linearity<1% from 0 to 3.0 OD typical
OD repeatability<0.5% at 2.0 OD typical
Reading speed96 wells: 11 seconds
384 wells: 22 seconds


Fluorescence Intensity

SensitivityFluorescein 2 pM typical (0.2 fmol/well 384-well plate) (Top)
Fluorescein 2.5 pM typical (0.25 fmol/well 384-well plate) (Bottom)
Light sourceHigh Energy Xenon Flash
Wavelength selectionDouble grating monochromators (Top and Bottom)
Wavelength range250-900 nm
Dynamic range5 decades
BandpassVariable: 9, 13.5, 17, 20 nm
Detection systemDedicated red shifted PMT
Reading speed96: 11 seconds
384: 22 seconds



Sensitivity10 amol ATP typical (flash)
Wavelength range300 - 700 nm
Dynamic range>6 decades
Detection systemLow noise PMT


Reagent Dispensers

Number2 syringe pumps
Dispense volume5 - 1000 µL in 1 µL increment
Dead volume1.1 mL, 100 µL with back flush


Physical Characteristics

Power250 Watts max
Dimensions17"W x 20.9"D x 15"H (43.5 x 53.1 x 38.1 cm)
Weight70 lbs (31.5 kg)



RegulatoryFor in vitro diagnostic use. All BioTek microplate instrumentation is CE and ETL marked. For details on approvals and standards compliance, please contact BioTek.


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