저널명 : Biosensors & Bioelectronics,119, 126-133 (2018) 
저자 : Rongke Gao*, Ziyi Cheng, Xiaokun Wang, Liandong Yu, Zhongyi Guo, Gang Zhao and Jaebum Choo* 
기타사항 :  

Abstract

The simultaneous detection of multiple biomarkers plays an important role in the accurate diagnosis of cancer. In this study, we developed a novel, surface-enhanced Raman scattering (SERS)-based microfluidic device for the simultaneous detection of free prostate-specific antigen (f-PSA) and total PSA (t-PSA) markers. A fully automatic droplet-based microfluidic platform for the rapid and sensitive detection of f-PSA and t-PSA was designed. Magnetic immunocomplexes were aligned on one side of the channel using a permanent magnet embedded in the microfluidic device, and parent microdroplets containing magnetic immunocomplexes and supernatant solutions were split into two smaller daughter droplets at the Y-shaped junction of the channel. Then, Raman signals of sequential droplets including supernatant solutions were measured for the quantitative analysis of the PSA markers. Two parallel microfluidic channels were designed and fabricated for the simultaneous detection of f-PSA and t-PSA. Our results showed a good linear response for both PSA markers in the range from 0.05 to 100 ng mL−1. The limits of detection were estimated to be below 0.1 ng mL−1 for both the f-PSA and t-PSA. This SERS-based assay in a microfluidic channel was completed in 10 min without any manual incubation and washing steps. Our method is a very promising clinical tool for PSA-based screening test of prostate cancer.


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List of Articles
제목 저널명 저자 기타사항

2018 Experimental Investigation of Surface Morphology of a Chemical Vapor Deposit...

저널명 Journal of Applied Physics, in press. 

저자 Won-Hwa Park,*, Minjung Kim, Hyeonsik Cheong and Jaebum Choo 

기타사항  

2019 High-throughput Microfluidic Imaging Flow Cytometry file

저널명 Current Opinion in Biotechnology, 55, 36-43 (2019) 

저자 Stavros Stavrakis, Gregor Holzner, Jaebum Choo* and Andrew deMello* 

기타사항  

2019 Modification of Phage Display Technique for Improved Screening of High-Aff...

저널명 Journal of Biotechnology, 289, 88-92 (2019) 

저자 Soi Yun, Sangah Lee, Jaebum Choo* and E. K. Lee* 

기타사항  

2018 Cut-and-Paste Transferrable Pressure Sensing Cartridge Films file

저널명 Chemistry of Materials, 30(18), 6410–6419 (2018). 

저자 H. Hwang, S. E. Choi, S. W. Han, I. You, E. S. Jeong, S. Kim, H. Yang, S. Lee, J. Choo, J. W. Kim* and U. Jeong* 

기타사항  

2018 A Sample-in-Digital-Answer-out System for Rapid Detection and Quantitation of... file

저널명 Analytical and Bioanalytical Chemistry, 410, 7019-7030 (2018). 

저자 H. Yang, Z. Chen, X. Cao, Z. Li, S. Stavrakis, J. Choo, A. J. deMello,* P. D. Howes and N. He* 

기타사항  

2018 Simultaneous immunoassays of dual prostate cancer markers using a SERS-based microdr... file

저널명 Biosensors & Bioelectronics,119, 126-133 (2018) 

저자 Rongke Gao*, Ziyi Cheng, Xiaokun Wang, Liandong Yu, Zhongyi Guo, Gang Zhao and Jaebum Choo* 

기타사항  

2018 An optofluidic system with integrated microlens arrays for parallel imaging flo... file

저널명 Lab on a Chip, 18, 3631-3637 (2018) 

저자 Gregor Holzner, Ying Du, Xiaobao Cao, Jaebum Choo, Andrew deMello* and Stavros Stavrakis* 

기타사항  

2018 Reinforcement Learning for Dynamic Microfluidic Control file

저널명 ACS Omega, 3, 10084-10091 (2018) 

저자 Oliver Dressler, Philip Howes, Jaebum Choo and Andrew deMelllo* 

기타사항  

2018 Application of SERS-based Microfluidics for In Vitro Diagnostics

저널명 In “Medical and Biological Applications Using Microfluidic Devices”, Book Chapter 9 (2018) in press, Springer. 

저자 Jinhyeok Jeon, Namhyun Choi, Joung-il Moon, Hao Chen and Jaebum Choo* 

기타사항 Book Chapter 

2018 Fluoropolymer Coated PDMS Microfluidic Devices for Application in Organic ... file

저널명 Chemistry - A European Journal, 24, 12078-12083 (2018) 

저자 T. Yang, J. Choo, S. Stavrakis* and A. deMello* 

기타사항  


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