저널명 : Lab on a Chip, 18, 3631-3637 (2018) 
저자 : Gregor Holzner, Ying Du, Xiaobao Cao, Jaebum Choo, Andrew deMello* and Stavros Stavrakis* 
기타사항 :  

Abstract

In recent years, high-speed imaging has become increasingly effective for the rapid analysis of single cells in flowing environments. Single cell imaging methods typically incorporate a minimum magnification of 10× when extracting sizing and morphological information. Although information content may be significantly enhanced by increasing magnification, this is accompanied by a corresponding reduction in field of view, and thus a decrease in the number of cells assayed per unit time. Accordingly, the acquisition of high resolution data from wide field views remains an unsolved challenge. To address this issue, we present an optofluidic flow cytometer integrating a refractive, microlens array (MLA) for imaging cells at high linear velocities, whilst maximizing the number of cells per field of view. To achieve this, we adopt an elasto-inertial approach for cell focusing within an array of parallel microfluidic channels, each equipped with a microlens. We characterize the optical performance of the microlenses in terms of image formation, magnification and resolution using both ray-tracing simulations and experimental measurements. Results demonstrate that the optofluidic platform can efficiently count and magnify micron-sized objects up to 4 times. Finally, we demonstrate the capabilities of the platform as an imaging flow cyclometer, demonstrating the efficient discrimination of hB and Jurkat cells at throughputs up to 50000 cells per second.


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List of Articles
제목 저널명 저자 기타사항

2018 Experimental Investigation of Surface Morphology of a Chemical Vapor Deposit...

저널명 Journal of Applied Physics, in press. 

저자 Won-Hwa Park,*, Minjung Kim, Hyeonsik Cheong and Jaebum Choo 

기타사항  

2019 High-throughput Microfluidic Imaging Flow Cytometry file

저널명 Current Opinion in Biotechnology, 55, 36-43 (2019) 

저자 Stavros Stavrakis, Gregor Holzner, Jaebum Choo* and Andrew deMello* 

기타사항  

2019 Modification of Phage Display Technique for Improved Screening of High-Aff...

저널명 Journal of Biotechnology, 289, 88-92 (2019) 

저자 Soi Yun, Sangah Lee, Jaebum Choo* and E. K. Lee* 

기타사항  

2018 Cut-and-Paste Transferrable Pressure Sensing Cartridge Films file

저널명 Chemistry of Materials, 30(18), 6410–6419 (2018). 

저자 H. Hwang, S. E. Choi, S. W. Han, I. You, E. S. Jeong, S. Kim, H. Yang, S. Lee, J. Choo, J. W. Kim* and U. Jeong* 

기타사항  

2018 A Sample-in-Digital-Answer-out System for Rapid Detection and Quantitation of... file

저널명 Analytical and Bioanalytical Chemistry, 410, 7019-7030 (2018). 

저자 H. Yang, Z. Chen, X. Cao, Z. Li, S. Stavrakis, J. Choo, A. J. deMello,* P. D. Howes and N. He* 

기타사항  

2018 Simultaneous immunoassays of dual prostate cancer markers using a SERS-based microdr... file

저널명 Biosensors & Bioelectronics,119, 126-133 (2018) 

저자 Rongke Gao*, Ziyi Cheng, Xiaokun Wang, Liandong Yu, Zhongyi Guo, Gang Zhao and Jaebum Choo* 

기타사항  

2018 An optofluidic system with integrated microlens arrays for parallel imaging flo... file

저널명 Lab on a Chip, 18, 3631-3637 (2018) 

저자 Gregor Holzner, Ying Du, Xiaobao Cao, Jaebum Choo, Andrew deMello* and Stavros Stavrakis* 

기타사항  

2018 Reinforcement Learning for Dynamic Microfluidic Control file

저널명 ACS Omega, 3, 10084-10091 (2018) 

저자 Oliver Dressler, Philip Howes, Jaebum Choo and Andrew deMelllo* 

기타사항  

2018 Application of SERS-based Microfluidics for In Vitro Diagnostics

저널명 In “Medical and Biological Applications Using Microfluidic Devices”, Book Chapter 9 (2018) in press, Springer. 

저자 Jinhyeok Jeon, Namhyun Choi, Joung-il Moon, Hao Chen and Jaebum Choo* 

기타사항 Book Chapter 

2018 Fluoropolymer Coated PDMS Microfluidic Devices for Application in Organic ... file

저널명 Chemistry - A European Journal, 24, 12078-12083 (2018) 

저자 T. Yang, J. Choo, S. Stavrakis* and A. deMello* 

기타사항  


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