저널명 : Analytical Chemistry, 89, 8413-8420 (2017) 
저자 : Namhyun Choi, Jiyoung Lee, Juhui Ko, Jun Ho Jeon, Gi-eun Rhie, Andrew deMello* and Jaebum Choo* 
기타사항 :  

Abstract

The development of surface-enhanced Raman scattering (SERS)-based microfluidic platforms has attracted significant recent attention in the biological sciences. SERS is a highly sensitive detection modality, with microfluidic platforms providing many advantages over microscale methods, including high analytical throughput, facile automation, and reduced sample requirements. Accordingly, the integration of SERS with microfluidic platforms offers significant utility in chemical and biological experimentation. Herein, we report a fully integrated SERS-based microdroplet platform for the automatic immunoassay of specific antigen fraction 1 (F1) in Yersinia pestis. Specifically, highly efficient and rapid immunoreactions are achieved through sequential droplet generation, transport, and merging, while wash-free immunodetection is realized through droplet-splitting. Such integration affords a novel multifunctional platform capable of performing complex multistep immunoassays in nL-volume droplets. The limit of detection of the F1 antigen for Yersinia pestis using the integrated SERS-based microdroplet platform is 59.6 pg/mL, a value approximately 2 orders of magnitude more sensitive than conventional enzyme-linked immunosorbent assays. This assay system has additional advantages including reduced sample consumption (less than 100 μL), rapid assay times (less than 10 min), and fully automated fluid control. We anticipate that this integrated SERS-based microdroplet device will provide new insights in the development of facile assay platforms for various hazardous materials.


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List of Articles
제목 저널명 저자 기타사항

2017 Integrated SERS-based microdroplet platform for the automated immunoassay of F1 ... file

저널명 Analytical Chemistry, 89, 8413-8420 (2017) 

저자 Namhyun Choi, Jiyoung Lee, Juhui Ko, Jun Ho Jeon, Gi-eun Rhie, Andrew deMello* and Jaebum Choo* 

기타사항  

2017 Analysis of ribonuclease activity in sub-nanoliter droplets by label-free fluo... file

저널명 Analyst, 142, 2610-2616 (2017) 

저자 Jae-Won Choi, Bala M. K. Vasamsetti, Kyu-Wan Kim, Seung Hwan Seo, Dong-Hun Lee, Soo-Ik Chang, Jaebum Choo* and Hak Yong Kim* 

기타사항  

2017 SERS-based genetic assay for amplification-free detection of prostate cancer ... file

저널명 Sensors & Actuators B, 251, 302-309 (2017) 

저자 Jimin Yu, Jinhyeok Jeon, Namhyun Choi, Jin Oh Lee, Young-Pil Kim and Jaebum Choo* 

기타사항  

2017 Simultaneous detection of dual prostate specific antigens using SERS-based immun... file

저널명 ACS Nano, 11, 4926-4933 (2017) 

저자 Ziyi Cheng, Namhyun Choi, Rui Wang, Sangyeop Lee, Kyung Chul Moon, Soo-Young Yoon, Lingxin Chen* and Jaebum Choo* 

기타사항  

2017 From single-molecule detection to next-generation sequencing: microfluidic dro... file

저널명 Microfluidics and Nanofluidics, 21:58 (2017) 

저자 Yun Ding, Jaebum Choo and Andrew deMello* 

기타사항  

2017 Fluorescent chemical probes for accurate tumor diagnosis and targeting therapy file

저널명 Chemical Society Reviews, 46, 2237-2271 (2017) 

저자 Min Gao, Fabiao Yu*, Changjun Lv, Jaebum Choo* and Lingxin Chen* 

기타사항  

2017 Quantum Dot–based Molecularly Imprinted Polymers on Three-Dimensional Origami ... file

저널명 ACS Sensors, 2, 243-250 (2017) 

저자 Bowei Li, Zhong Zhang, Ji Qi, Na Zhou, Song Qin, Jaebum Choo* and Lingxin Chen* 

기타사항  

2017 Spectroscopic measurements of interactions between hydrophobic 1-pyrenebutyric... file

저널명 Colloids and Surfaces A, 518, 295-303 (2017) 

저자 Nguyen Hoang Ly, Thanh Danh Nguyen, Thanh Lam Bui, Sangyeop Lee, Jaebum Choo, Sang-Woo Joo* 

기타사항  

2017 Raman spectrum identification based on the correlation score using weighted ... file

저널명 Analyst, 142, 380-388 (2017) 

저자 Jun-Kyu Park, Aaron Park, Si Kyung Yang, Sung-June Baek*, Joonki Hwang and Jaebum Choo* 

기타사항  

2017 Simultaneous Detection of Dual Nucleic Acids Using a SERS-based Lateral Flow... file

저널명 Analytical Chemistry, 89, 1163-1169 (2017) 

저자 Xiaokun Wang, Namhyun Choi, Ziyi Cheng, Juhui Ko, Lingxin Chen*, and Jaebum Choo* 

기타사항  


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