저널명 : Sensors & Actuators B, 251, 302-309 (2017) 
저자 : Jimin Yu, Jinhyeok Jeon, Namhyun Choi, Jin Oh Lee, Young-Pil Kim and Jaebum Choo* 
기타사항 :  

Abstract

We report the development of amplification-free surface-enhanced Raman scattering (SERS)-based DNA assays for the rapid and highly sensitive detection of prostate cancer antigen 3 (PCA3) mimic DNA. This technique does not require any DNA amplification process using thermo-cycles in conventional polymerase chain reaction (PCR) due to its highly sensitive detection capability. Herein, the PCA3 mimic DNA, composed of 45 nucleotide sequences, was sandwiched between two probe DNA-immobilized particles (ASO735-conjugated detection HGNs and ASO683-immobilized capture magnetic beads) by hybridization reactions. Its quantitative analysis was performed by monitoring the characteristic Raman peak intensity of sandwich DNA complexes. The limit of detection (LOD) is estimated to be 2.7 fM, which is about four orders of magnitude more sensitive than that of conventional PCR. This SERS-based DNA assay technique is expected to be a potentially useful tool for early disease diagnosis.


Download PDF = Click Attachment, please.

List of Articles
제목 저널명 저자 기타사항

2017 An integrated SERS-based microdroplet platform for the automated immunoassay of...

저널명 Analytical Chemistry, in press. 

저자 Namhyun Choi, Jiyoung Lee, Juhui Ko, Jun Ho Jeon, Gi-eun Rhie, Andrew deMello* and Jaebum Choo* 

기타사항  

2017 Analysis of ribonuclease activity in sub-nanoliter droplets by label-free fluo... file

저널명 Analyst, 142, 2610-2616 (2017) 

저자 Jae-Won Choi, Bala M. K. Vasamsetti, Kyu-Wan Kim, Seung Hwan Seo, Dong-Hun Lee, Soo-Ik Chang, Jaebum Choo* and Hak Yong Kim* 

기타사항  

2017 SERS-based genetic assay for amplification-free detection of prostate cancer ... file

저널명 Sensors & Actuators B, 251, 302-309 (2017) 

저자 Jimin Yu, Jinhyeok Jeon, Namhyun Choi, Jin Oh Lee, Young-Pil Kim and Jaebum Choo* 

기타사항  

2017 Simultaneous detection of dual prostate specific antigens using SERS-based immun... file

저널명 ACS Nano, 11, 4926-4933 (2017) 

저자 Ziyi Cheng, Namhyun Choi, Rui Wang, Sangyeop Lee, Kyung Chul Moon, Soo-Young Yoon, Lingxin Chen* and Jaebum Choo* 

기타사항  

2017 From single-molecule detection to next-generation sequencing: microfluidic dro... file

저널명 Microfluidics and Nanofluidics, 21:58 (2017) 

저자 Yun Ding, Jaebum Choo and Andrew deMello* 

기타사항  

2017 Fluorescent chemical probes for accurate tumor diagnosis and targeting therapy file

저널명 Chemical Society Reviews, 46, 2237-2271 (2017) 

저자 Min Gao, Fabiao Yu*, Changjun Lv, Jaebum Choo* and Lingxin Chen* 

기타사항  

2017 Quantum Dot–based Molecularly Imprinted Polymers on Three-Dimensional Origami ... file

저널명 ACS Sensors, 2, 243-250 (2017) 

저자 Bowei Li, Zhong Zhang, Ji Qi, Na Zhou, Song Qin, Jaebum Choo* and Lingxin Chen* 

기타사항  

2017 Spectroscopic measurements of interactions between hydrophobic 1-pyrenebutyric... file

저널명 Colloids and Surfaces A, 518, 295-303 (2017) 

저자 Nguyen Hoang Ly, Thanh Danh Nguyen, Thanh Lam Bui, Sangyeop Lee, Jaebum Choo, Sang-Woo Joo* 

기타사항  

2017 Raman spectrum identification based on the correlation score using weighted ... file

저널명 Analyst, 142, 380-388 (2017) 

저자 Jun-Kyu Park, Aaron Park, Si Kyung Yang, Sung-June Baek*, Joonki Hwang and Jaebum Choo* 

기타사항  

2017 Simultaneous Detection of Dual Nucleic Acids Using a SERS-based Lateral Flow... file

저널명 Analytical Chemistry, 89, 1163-1169 (2017) 

저자 Xiaokun Wang, Namhyun Choi, Ziyi Cheng, Juhui Ko, Lingxin Chen*, and Jaebum Choo* 

기타사항  


XE Login